Protein_Domain

Part:BBa_K3280000

Designed by: Amanda Araujo Silva ; Raquel González Sterman ; André Gustavo Jucovski ; Juliana Naomi Yamauti Costa ; Roberto Hiroshi Matos Furuta; João Paulo Cassucci dos Santos   Group: iGEM19_USP_SaoCarlos-Brazil   (2019-10-15)


RBS + tDGC (from Thermotoga maritima)

tDGC. This part is a protein domain and increase levels of c-di-GMP to induce biofilm formation.

Usage and Biology

This part is originated from Thermatoga maritma.

Cyclic di-GMP (c-di-GMP) is recognized as an intracellular signaling molecule coordinating the “lifestyle transition†from motility to sessility and vice versa (i.e. dispersion). The correlation between high c-di-GMP concentration in the cell and biofilm formation or between low c-di-GMP levels and motility has been demonstrated in several bacteria species [1].


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 238
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 601
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI site found at 102


[edit]
Categories
Parameters
None